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Biochim Biophys Acta. 1997 Oct 17;1342(2):119-31.

Psychrophilic enzymes: a thermodynamic challenge.

Author information

1
Laboratory of Biochemistry, Institute of Chemistry, University of Li├Ęge, Belgium. ch.gerday@ulg.ac.be

Abstract

Psychrophilic microorganisms, hosts of permanently cold habitats, produce enzymes which are adapted to work at low temperatures. When compared to their mesophilic counterparts, these enzymes display a higher catalytic efficiency over a temperature range of roughly 0-30 degrees C and a high thermosensitivity. The molecular characteristics of cold enzymes originating from Antarctic bacteria have been approached through protein modelling and X-ray crystallography. The deduced three-dimensional structures of cold alpha-amylase, beta-lactamase, lipase and subtilisin have been compared to their mesophilic homologs. It appears that the molecular adaptation resides in a weakening of the intramolecular interactions, and in some cases in an increase of the interaction with the solvent, leading to more flexible molecular edifices capable of performing catalysis at a lower energy cost.

PMID:
9392521
DOI:
10.1016/s0167-4838(97)00093-9
[Indexed for MEDLINE]

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