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Acta Cytol. 1997 Nov-Dec;41(6):1742-6.

Urine cytology and the diagnosis of renal allograft rejection. II. Studies using immunostaining.

Author information

1
Department of Pediatrics, Montefiore Medical Center/Albert Einstein College of Medicine, Bronx, New York, USA.

Abstract

BACKGROUND:

Urine immunocytology may provide a noninvasive method of investigating the antigens expressed by renal tubular cells. In previous investigations of patients with acute renal allograft rejection (AR), we showed that the adhesion molecule ICAM-1 is expressed by voided tubular cells. The up-regulation of ICAM-1, in turn, may be due to high circulating levels of interferon-gamma and/or TNF-alpha. We investigated the regulation of receptors for these cytokines and found a correlation between their expression and clinical events.

STUDY DESIGN:

For 10 patients who received transplants consecutively, freshly voided aliquots of urine were obtained on each hospital day and on each outpatient visit for a mean of 52.8 +/- 26.2 (SD) days. After cytocentrifugation, the samples were prepared by the avidin-biotin-immunoperoxidase technique in order to detect the presence or absence of ICAM-1, interferon-gamma receptor and TNF-alpha receptor (p 80) on the tubular cells.

RESULTS:

In nonrejecting patients, the tubular cells expressed the interferon-gamma receptor but not ICAM-1 or the TNF-alpha receptor. In patients with AR, the pattern was different. The tubular cells expressed ICAM-1 and the TNF-alpha receptor but not the interferon-gamma receptor.

CONCLUSION:

Urine immunocytochemistry may be useful to demonstrate the expression of cytokine receptors by renal epithelia.

PMID:
9390134
DOI:
10.1159/000333178
[Indexed for MEDLINE]

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