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J Virol Methods. 1997 Nov;68(2):193-8.

Propagation and titration of murine cytomegalovirus in a continuous bone marrow-derived stromal cell line (M2-10B4).

Author information

1
Department of Medicine, University of Minnesota Medical School, Minneapolis 55455, USA.

Abstract

Murine cytomegalovirus (MCMV) can only be propagated effectively in mouse embryo fibroblast (MEF) cells. We demonstrate that MCMV replicates significantly better in M2-10B4 cells, a continuous line of murine bone marrow stromal cells. M2-10B4 cells were also comparable to MEF cells for detection of small amounts of MCMV reactivating from latently infected spleen explants. M2-10B4 cells will be very useful for studies of MCMV pathogenesis.

PMID:
9389409
DOI:
10.1016/s0166-0934(97)00126-2
[Indexed for MEDLINE]

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