Production and refolding of recombinant leptin

Biotechniques. 1997 Nov;23(5):800-2, 804. doi: 10.2144/97235bm07.
No abstract available

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adipose Tissue / chemistry
  • Animals
  • Base Sequence
  • Body Weight / drug effects
  • Chromatography, Affinity
  • Cloning, Molecular
  • DNA, Complementary / analysis
  • Escherichia coli / genetics
  • Female
  • Gene Expression
  • Leptin
  • Mice
  • Mice, Obese
  • Plasmids / genetics
  • Polymerase Chain Reaction
  • Protein Biosynthesis*
  • Protein Folding*
  • Proteins / chemistry
  • Proteins / genetics
  • RNA / isolation & purification
  • RNA-Directed DNA Polymerase
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / pharmacology

Substances

  • DNA, Complementary
  • Leptin
  • Proteins
  • Recombinant Proteins
  • RNA
  • RNA-Directed DNA Polymerase