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Curr Biol. 1997 Nov 1;7(11):850-9.

Translational attenuation mediated by an mRNA intron.

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Howard Hughes Medical Institute, Department of Biochemistry and Biophysics, University of California School of Medicine, San Francisco 94143-0448, USA.



The unfolded protein response (UPR) is an intracellular signaling pathway that is activated by the accumulation of unfolded proteins in the endoplasmic reticulum (ER). The UPR results in an increase in transcription of ER-resident proteins that facilitate protein folding in the ER. A key regulatory step in UPR activation is the regulated splicing of HAC1 mRNA, which encodes Hac1p, a transcription factor dedicated to this pathway. Hac1p can be detected only when the spliced form of HAC1 mRNA (termed HAC1i mRNA, for induced) is produced; this was surprising because the unspliced HAC1u mRNA (HAC1u for uninduced) is equally stable in cells.


We show that in contrast to most other unspliced pre-mRNAs, the HAC1u mRNA is transported from the nucleus into the cytosol. Although HAC1u mRNA is associated with polyribosomes, no detectable Hac1pu is produced unless the intron is removed, indicating that the presence of the intron prevents mRNA translation. When it is produced, Hac1pu has a stability similar to that of Hac1pi, the form of the Hac1p that is produced from the spliced mRNA and that differs from Hac1pu by a short carboxy-terminal tail sequence. Hac1pu, however, is differently modified and less active in activating transcription. Interestingly, when transplanted into the 3' untranslated region of a completely unrelated mRNA, the HAC1 intron is sufficient to attenuate translation of the preceding open reading frame.


We have shown that the HAC1 mRNA intron is both necessary and sufficient to prevent complete translation of polyribosome-associated mRNAs. To our knowledge, this identifies a new way by which translation of a mRNA can be attenuated.

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