Galactosylation with beta-galactosidase from bovine testes employing modified acceptor substrates

U Gambert; R Gonzalez Lio; E Farkas; J Thiem; V Verez Bencomo; A Lipták

doi:10.1016/s0968-0896(97)00071-0

Galactosylation with beta-galactosidase from bovine testes employing modified acceptor substrates

Bioorg Med Chem. 1997 Jul;5(7):1285-91. doi: 10.1016/s0968-0896(97)00071-0.

Authors

U Gambert¹, R Gonzalez Lio, E Farkas, J Thiem, V Verez Bencomo, A Lipták

Affiliation

¹ Institut für Organische Chemie, Universität Hamburg, Germany.

PMID: 9377088
DOI: 10.1016/s0968-0896(97)00071-0

Abstract

In this study beta1-3 linked analogues of the T-antigen determinant were synthesized in preparative scale by transgalactosylation using beta-galactosidase from bovine testes to give synthetic antigens. Acceptors with modifications of the sugar residue such as alpha-glycosylated spacers, as well as GlcNAc-alphaOR- and 2dGal-alphaOR-substrates opened further possibilities for galactosylation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens, Viral, Tumor / chemistry*
Cattle
Disaccharides / chemical synthesis*
Epitopes / chemistry
Galactosides / chemical synthesis*
Glycosylation
Male
Substrate Specificity
Testis / enzymology*
beta-Galactosidase / metabolism*

Substances

Antigens, Viral, Tumor
Disaccharides
Epitopes
Galactosides
beta-Galactosidase