DNA-probes for the differentiation of Capnocytophaga species

G Conrads; R Mutters; I Seyfarth; K Pelz

doi:10.1006/mcpr.1997.0124

DNA-probes for the differentiation of Capnocytophaga species

Mol Cell Probes. 1997 Oct;11(5):323-8. doi: 10.1006/mcpr.1997.0124.

Authors

G Conrads¹, R Mutters, I Seyfarth, K Pelz

Affiliation

¹ Clinic of Conservative & Preventive Dentstry and Periodontology, University Hospital (RWTH) Aachen, Germany.

PMID: 9375291
DOI: 10.1006/mcpr.1997.0124

Abstract

We designed oligonucleotides to differentiate between the seven currently known Capnocytophaga species. The oligonucleotides were labelled non-radioactively at the 3' end with digoxigenin. The specificity could be demonstrated in a dot-blot hybridization assay by using the type strains, reference strains, and 37 clinical Capnocytophaga isolates as well as 11 representative strains of other taxa as a template. The sensitivity of the assay was calculated with 10(3) bacteria per dot.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques*
Capnocytophaga / classification*
Capnocytophaga / genetics
DNA Probes*
DNA, Bacterial / genetics
DNA, Ribosomal / genetics
Digoxigenin
Nucleic Acid Hybridization
Oligodeoxyribonucleotides / chemistry
RNA, Ribosomal, 16S / genetics
Sensitivity and Specificity
Species Specificity

Substances

DNA Probes
DNA, Bacterial
DNA, Ribosomal
Oligodeoxyribonucleotides
RNA, Ribosomal, 16S
Digoxigenin