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J Vet Med Sci. 1997 Oct;59(10):935-7.

Genetic analysis of equine methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis.

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Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University, Hyogo, Japan.


Pulsed-field gel electrophoresis (PFGE) was used to determine genetic relationships among 15 methicillin-resistant Staphylococcus aureus (MRSA) isolates from mares with metritis and from a stallion with dermatitis in Hokkaido. All the 15 isolates showed phage pattern 6/47/54/75, coagulase type IV, and enterotoxin type A. The restriction endonuclease SmaI cut their genomic DNAs into 15 or 16 fragments ranging in size from 8 to 630 kb. Fourteen of the 15 isolates showed the same PFGE pattern, whereas the remaining one appeared to be closely related. The 9 human MRSA isolates showing the same phenotypic characteristics as the horse isolates gave different PFGE patterns from those of the horse isolates.

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