Format

Send to

Choose Destination
Am J Pathol. 1997 Nov;151(5):1273-80.

Serial transplantation reveals the stem-cell-like regenerative potential of adult mouse hepatocytes.

Author information

1
Department of Molecular and Medical Genetics, Oregon Health Sciences University, Portland 97201, USA.

Abstract

Previous work has shown that adult mouse hepatocytes can divide at least 18 times in vivo. To test whether this represents the upper limit of their regenerative capacity, we performed serial transplantation of hepatocytes in the fumarylacetoacetate hydrolase deficiency murine model of liver repopulation. Hepatocytes from adult donors were serially transplanted in limiting numbers six times and resulted in complete repopulation during each cycle. This corresponds to a minimal number of 69 cell doublings or a 7.3 x 10(20)-fold expansion. No evidence for abnormal liver function or altered hepatic architecture was found in repopulated animals. We conclude that a fraction of adult mouse hepatocytes have growth potential similar to that of hematopoietic stem cells.

PMID:
9358753
PMCID:
PMC1858091
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for PubMed Central
Loading ...
Support Center