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Gene. 1997 Oct 15;199(1-2):173-9.

Tissue-specific alternative RNA splicing of rat vesicle-associated membrane protein-1 (VAMP-1).

Author information

1
Department of Medicine and the Digestive Disease Center, Stanford University School of Medicine, CA 94305-5487, USA.

Abstract

The vesicle-associated membrane protein (VAMP) family is essential to vesicle-mediated protein transport. Three mammalian isoforms, VAMP-1, VAMP-2, and cellubrevin, play a role in protein transport to the plasma membrane. In this study, we describe a new rat VAMP-1 isoform produced by alternative pre-mRNA splicing. Only one VAMP-1 isoform dominates in each tissue. Analysis of the nucleotide sequence for the newly discovered isoform, VAMP-1b, reveals that its expression is determined by whether an intron is retained or removed. The predicted amino acid sequences for the VAMP-1 isoforms differ at the carboxy-terminal end of the protein. A similar process has been described for VAMPs in Drosophila melanogaster and suggests a conserved function for the carboxy-terminal domain that can be modulated.

PMID:
9358054
[Indexed for MEDLINE]

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