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Gene. 1997 Oct 15;199(1-2):133-7.

Direct cloning of the Brassica S locus by using a P1-derived artificial chromosome (PAC) vector.

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Laboratory of Plant Breeding, Faculty of Agriculture, Tohoku University, Sendai, Japan.


Self-incompatibility of Brassica is regulated by the S locus, which contains several genes including SLG and SRK. We found that both SLG and SRK genes were located at an approx. 80-kb MluI fragment in an S9 haplotype of B. campestris. Therefore, we cloned this MluI fragment into a BssHII site of the P1-derived artificial chromosome (PAC) vector. The utility of the direct cloning method is discussed in this study.

[Indexed for MEDLINE]

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