Format

Send to

Choose Destination
J Mol Biol. 1997 Oct 31;273(3):614-22.

A conformational transition at the N terminus of the prion protein features in formation of the scrapie isoform.

Author information

1
Department of Neurology, School of Pharmacy, University of California, San Francisco, CA 94143, USA.

Abstract

The scrapie prion protein (PrPSc) is formed from the cellular isoform (PrPC) by a post-translational process that involves a profound conformational change. Linear epitopes for recombinant antibody Fab fragments (Fabs) on PrPC and on the protease-resistant core of PrPSc, designated PrP 27-30, were identified using ELISA and immunoprecipitation. An epitope region at the C terminus was accessible in both PrPC and PrP 27-30; in contrast, epitopes towards the N-terminal region (residues 90 to 120) were accessible in PrPC but largely cryptic in PrP 27-30. Denaturation of PrP 27-30 exposed the epitopes of the N-terminal domain. We argue from our findings that the major conformational change underlying PrPSc formation occurs within the N-terminal segment of PrP 27-30.

PMID:
9356250
DOI:
10.1006/jmbi.1997.1328
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center