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Biochim Biophys Acta. 1997 Sep 12;1353(3):199-202.

An Arabidopsis gene encoding a putative 14-3-3-interacting protein, caffeic acid/5-hydroxyferulic acid O-methyltransferase.

Author information

1
Department of Biological Sciences, Texas Tech University, Lubbock 79409, USA. brahz@ttacs.ttu.edu

Abstract

AFT1, a 14-3-3 protein from Arabidopsis thaliana, was used as a 'bait' in the two-hybrid system to identify its interacting proteins. A caffeic acid/5-hydroxyferulic acid O-methyltransferase, OMT1, was identified as one of the several proteins that specifically interacts with AFT1 in yeast cells. The physical interaction between AFT1 and a partial OMT1 polypeptide can be demonstrated in vitro by using bacterially expressed proteins. A single copy gene was found to encode OMT1 in Arabidopsis, and its expression is both spatially and temporally regulated.

PMID:
9349713
DOI:
10.1016/s0167-4781(97)00096-1
[Indexed for MEDLINE]

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