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Eur J Pharmacol. 1997 Sep 3;334(1):49-53.

Contribution of endopeptidase to central neurotensin inactivation.

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Institut de pharmacologie mol├ęculaire et Cellulaire du CNRS, UPR411, Valbonne, France.


The tridecapeptide, neurotensin elicits naloxone-insensitive analgesia after its intracebroventricular administration in mice. We used this central pharmacological effect to assess the putative contribution of the endopeptidase to central inactivation of the peptide. By means of combinatorial chemistry, we previously designed the first potent endopeptidase inhibitor. This agent, Z-(L,D)Phe psi(PO2CH2)(L,D)Ala-Lys-Met (phosphodiepryl 21), is shown here to behave as a fully specific endopeptidase inhibitor, as demonstrated by the absence of effect on a series of other exo- and endopeptidases belonging to various classes of proteolytic activities present in murine brain membranes. Furthermore, central administration of phosphodiepryl 21 drastically prolongs the forepaw licking latency of mice tested on the hot plate and injected with sub-maximally active doses of neurotensin. Altogether, our results demonstrated that, in addition to endopeptidase, endopeptidase likely contributes to the physiological termination of the neurotensinergic message in murine brain.

[Indexed for MEDLINE]

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