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Exp Cell Res. 1997 Oct 10;236(1):201-11.

Dynamic organization of DNA replication in one-cell mouse embryos: relationship to transcriptional activation.

Author information

1
Laboratoire Associé Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA), Paris, France. bouniol@.ibpc.fr

Abstract

We have analyzed the spatial and temporal relationship between transcription and replication sites during the first cell cycle in mouse embryos. Embryos were microinjected with both 5-bromouridine-5'-triphosphate and digoxygenin-11-deoxyuridine-5'-triphosphate to visualize transcription and replication sites respectively. We detected six different phases of replication during S phase and dated the onset of zygotic transcription at the end of the S phase. Using confocal microscopy, we showed that there is essentially no colocalization of replication and transcription sites at this stage of development. Moreover, studies on aphidicolin-treated embryos demonstrated that inhibition of DNA replication does not hinder transcriptional activation at the 1-cell stage.

PMID:
9344600
DOI:
10.1006/excr.1997.3708
[Indexed for MEDLINE]

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