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Methods Enzymol. 1997;287:162-74.

Expression of chemokine RANTES and production of monoclonal antibodies.

Author information

1
Department of Pediatrics, Stanford University Medical Center, California 94305-5119, USA.

Abstract

RANTES was first identified as a cDNA in a search for genes expressed late (3-5 days) after T-cell activation. Definition of RANTES function depended on the generation of protein. This chapter describes the various techniques used to make recombinant RANTES protein, to test its activity, and to generate monoclonal antibodies to assess RANTES protein cell distribution.

PMID:
9330321
DOI:
10.1016/s0076-6879(97)87013-9
[Indexed for MEDLINE]

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