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J Immunol Methods. 1997 Aug 7;206(1-2):53-9.

An ELISA for hCAP-18, the cathelicidin present in human neutrophils and plasma.

Author information

1
Department of Hematology, Finsen Center, Rigshospitalet, Copenhagen, Denmark.

Abstract

hCAP-18 is a newly described protein of human neutrophilic granulocytes which belongs to the cathelicidin family of antimicrobial proteins. Members of this protein family share a common N-terminal sequence followed by a highly diverse antimicrobial, cationic C-terminus. The present work describes the production of recombinant hCAP-18, the generation of antibodies to the protein and the development of an accurate, sensitive and specific ELISA for the detection of hCAP-18 in cells, plasma and urine with a detection limit of 0.084 ng/ml. The amount of hCAP-18 in neutrophils is 0.627 microgram protein per 10(6) cells. The plasma level is 1.18 micrograms/ml which is several fold higher than for other neutrophil specific granule proteins. hCAP-18 is present in plasma as high molecular weight complexes. In accordance with this, hCAP-18 is barely excreted in the urine. The bone marrow appears to be the major source of plasma hCAP-18. The high level of hCAP-18 in plasma may provide an important defense against microorganisms and endotoxins.

PMID:
9328568
DOI:
10.1016/s0022-1759(97)00084-7
[Indexed for MEDLINE]

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