Format

Send to

Choose Destination
FEBS Lett. 1997 Sep 15;414(3):557-61.

Properties of the extracellular calcium binding module of the proteoglycan testican.

Author information

1
Max-Planck-Institut für Biochemie, Martinsried, Germany.

Abstract

The extracellular calcium-binding (EC) module of human testican (115 residues) was obtained in native form by recombinant production in mammalian cell culture and thus shown to represent an independently folding domain. This module showed a large loss in alpha-helix upon calcium depletion. Apparently only one of the two EF hands binds calcium, with a moderate affinity (Kd =68 microM) about 100-fold lower than in the homologous BM-40 protein. No clear evidence was obtained for collagen binding, indicating that EC modules found in different proteins may not share similar functions.

PMID:
9323035
DOI:
10.1016/s0014-5793(97)01070-3
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Wiley
Loading ...
Support Center