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FEMS Microbiol Lett. 1997 Sep 15;154(2):389-96.

Cloning of the aceF gene encoding the phosphomannose isomerase and GDP-mannose pyrophosphorylase activities involved in acetan biosynthesis in Acetobacter xylinum.

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1
Institute of Food Research, Norwich Laboratory, Colney, UK. annette.griffin@bbsrc.ac.uk

Abstract

The aceF gene from Acetobacter xylinum was identified and cloned from a genomic DNA library. The complete DNA sequence was determined and computer analysis of the translated gene sequence revealed homology with the deduced amino acid sequence of xanB from Xanthomonas campestris. Therefore aceF is likely to encode a bifunctional enzyme with mannose-6-phosphate isomerase (PMI) and GDP-mannose pyrophosphorylase (GMP) activities. PMI and GMP activities were detected in strains of Escherichia coli expressing the cloned aceF gene.

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