Abstract
We have identified three avian (chicken) Hu/elav family RNA-binding protein genes. cHuD and cHuC are expressed specifically in neurons of both the central and peripheral nervous systems. Although cHuA is expressed in a wide variety of tissues, including neurogenic precursor cells, it is transiently down-regulated, and is then re-expressed in maturing neurons. Misexpression of cHuD in cultured neural crest cells results in a dramatic increase in the proportion of cells exhibiting neuronal morphology, molecular markers for neurons, and neurotrophin dependence. These data confirm that cHuD protein is involved in regulating neuronal differentiation.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3T3 Cells
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Amino Acid Sequence
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Animals
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Apoptosis
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Cell Differentiation
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Cell Division
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Cells, Cultured
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Chick Embryo
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Coturnix
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ELAV Proteins
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Gene Expression Regulation, Developmental
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Genetic Markers
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Humans
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In Situ Hybridization
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Mice
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics*
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Nerve Tissue Proteins / metabolism*
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Nervous System / cytology
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Nervous System / embryology*
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Nervous System / metabolism*
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Neural Crest / cytology
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Neural Crest / metabolism
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Neurons / cytology
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Neurons / metabolism
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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RNA-Binding Proteins / genetics*
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RNA-Binding Proteins / metabolism*
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Sequence Homology, Amino Acid
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Species Specificity
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Transfection
Substances
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ELAV Proteins
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Genetic Markers
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Nerve Tissue Proteins
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RNA, Messenger
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RNA-Binding Proteins