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EMBO J. 1997 Aug 1;16(15):4560-7.

Reconstitution of the protein insertion machinery of the mitochondrial inner membrane.

Author information

1
Department of Biochemistry, Biozentrum, University of Basel, Switzerland. volker.haucke@yale.edu

Erratum in

  • EMBO J 1997 Oct 1;16(19):6055.

Abstract

We have reconstituted the protein insertion machinery of the yeast mitochondrial inner membrane into proteoliposomes. The reconstituted proteoliposomes have a distinct morphology and protein composition and correctly insert the ADP/ATP carrier (AAC) and Tim23p, two multi-spanning integral proteins of the mitochondrial inner membrane. The reconstituted system requires a membrane potential, but not Tim44p or mhsp70, both of which are required for the ATP-driven translocation of proteins into the matrix. The protein insertion machinery can thus operate independently of the energy-transducing Tim44p-mhsp70 complex.

PMID:
9303300
PMCID:
PMC1170082
DOI:
10.1093/emboj/16.15.4560
[Indexed for MEDLINE]
Free PMC Article
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