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J Chromatogr B Biomed Sci Appl. 1997 Aug 1;695(2):444-7.

High-performance liquid chromatographic determination of 4-methylpyrazole in plasma and in dialysate.

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Laboratoire de Pharmacologie et Toxicologie, C.H.U., Angers, France.


A rapid method for the determination of 4-methylpyrazole (4-MP) levels in plasma and in dialysate by isocratic reversed-phase high-performance liquid chromatography with UV detection is described. The internal standard was the 3-methylpyrazole (3-MP). Plasma sample preparation consisted of a protein precipitation. Dialysate samples were injected without preparation. The method was linear up to 30 mg l(-1) in plasma and up to 5 mg l(-1) in dialysate. The within-day precisions (C.V.) were less than 4% in plasma and were less than 2% in dialysate. The day-to-day precisions (C.V.) were less than 7% in plasma and were less than 3% in dialysate. This method is easy to perform and has practical interest for clinicians who need to monitor in emergency 4-MP levels in ethylene glycol and methanol poisonings.

[Indexed for MEDLINE]

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