Format

Send to

Choose Destination
Fungal Genet Biol. 1997 Jun;21(3):278-91.

AFLP Linkage Map of the Oomycete Phytophthora infestans

Author information

1
Graduate School Experimental Plant Sciences, Wageningen Agricultural University, Binnenhaven 9, Wageningen, 6709 PD, The Netherlands

Abstract

Here we present the first comprehensive genetic linkage map of the heterothallic oomycetous plant pathogen Phytophthora infestans. The map is based on polymorphic DNA markers generated by the DNA fingerprinting technique AFLP (Vos et al., 1995, Nucleic Acids Res. 23: 4407-4414). AFLP fingerprints were made from single zoospore progeny and 73 F1 progeny from two field isolates of P. infestans. The parental isolates appeared to be homokaryotic and diploid, their AFLP patterns were mitotically stable, and segregation ratios in the F1 progeny were largely Mendelian. In addition to 183 AFLP markers, 7 RFLP markers and the mating type locus were mapped. The linkage map comprises 10 major and 7 minor linkage groups covering a total of 827 cM. The major linkage groups are composed of markers derived from both parents, whereas the minor linkage groups contain markers from either the A1 or the A2 mating type parent. Non-Mendelian segregation ratios were found for the mating type locus and for 13 AFLP markers, all of which are located on the same linkage group as the mating type locus.

PMID:
9299198
DOI:
10.1006/fgbi.1997.0981

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center