[Comparative analysis of expression of genes for 2',5'-oligoadenylate synthetase and mRNA for dsRNA-protein kinase in human fibroblasts infected with the Sindbis alphavirus and induced by alpha-interferon]

T M Sokolova; O V Bobrova; A Iu Lebedev; N S Bystrov; L V Uryvaev

[Comparative analysis of expression of genes for 2',5'-oligoadenylate synthetase and mRNA for dsRNA-protein kinase in human fibroblasts infected with the Sindbis alphavirus and induced by alpha-interferon]

Vopr Virusol. 1997 May-Jun;42(3):98-102.

[Article in Russian]

Authors

T M Sokolova, O V Bobrova, A Iu Lebedev, N S Bystrov, L V Uryvaev

PMID: 9297349

Abstract

Expression of 2,5-OAS and ds-protein kinase (ds-PK) in human fibroblast culture following treatment with alpha-IF in a dose of 1000 U or challenge with Karelian fever virus (KFV) (5 PFU/cell, 4 h.p.i.) was compared. A highly sensitive and rapid method, coupled RT-PCR, was used to detect mRNA transcription levels. Alpha-IF had an expressed stimulating effect on 2,5-OAS mRNA level, whereas the virus markedly inhibited the synthesis of ds-PK mRNA.

Publication types

Comparative Study

MeSH terms

2',5'-Oligoadenylate Synthetase / genetics*
Fibroblasts / drug effects
Fibroblasts / enzymology
Fibroblasts / virology
Gene Expression Regulation, Enzymologic*
Humans
Interferon-alpha / pharmacology*
Polymerase Chain Reaction
Protein Serine-Threonine Kinases / genetics*
RNA, Messenger / genetics
Sindbis Virus / physiology*
Transcription, Genetic
eIF-2 Kinase

Substances

Interferon-alpha
RNA, Messenger
Protein Serine-Threonine Kinases
eIF-2 Kinase
2',5'-Oligoadenylate Synthetase