Location-specific regulation of transgenic Ly49A receptors by major histocompatibility complex class I molecules

Eur J Immunol. 1997 Aug;27(8):2057-65. doi: 10.1002/eji.1830270833.

Abstract

Inhibitory receptors expressed on natural killer (NK) cells and T cells specific for major histocompatibility complex (MHC) class I are believed to prevent these cells from responding to normal self tissues. To understand the regulation and function of Ly49 receptor molecules in vivo, we used the CD2 promoter to target Ly49A expression to all thymocytes, T cells, and NK cells. In animals expressing its MHC class I ligand, H-2Dd or H-2Dk, there was a large decrease in the expression of Ly49A on thymocytes, peripheral T cells, and NK1.1+ cells. The extent of the down-regulation of Ly49A was dependent on the expression of the MHC ligand for Ly49A and on the site where the cells were located. The level of expression of endogenous Ly49A was similarly found to be dependent upon the organ where the cells resided. Data from bone marrow chimeras indicated that most cell types may regulate Ly49A expression, but the efficacy to regulate receptor expression may vary depending on the cell type.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Ly*
  • Cells, Cultured
  • Concanavalin A / pharmacology
  • Down-Regulation
  • Female
  • Gene Expression
  • H-2 Antigens / genetics
  • H-2 Antigens / metabolism*
  • Hematopoietic System / cytology
  • Hematopoietic System / immunology
  • Hematopoietic System / metabolism
  • Histocompatibility Antigen H-2D
  • Killer Cells, Natural / immunology
  • Killer Cells, Natural / metabolism
  • Lectins, C-Type
  • Male
  • Membrane Glycoproteins / immunology*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • NK Cell Lectin-Like Receptor Subfamily A
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Receptors, Immunologic / genetics
  • Receptors, Immunologic / metabolism*
  • Receptors, NK Cell Lectin-Like
  • T-Lymphocytes / immunology
  • T-Lymphocytes / metabolism
  • Thymus Gland / cytology
  • Thymus Gland / immunology
  • Thymus Gland / metabolism
  • Tissue Distribution

Substances

  • Antigens, Ly
  • H-2 Antigens
  • Histocompatibility Antigen H-2D
  • Klra1 protein, mouse
  • Lectins, C-Type
  • Membrane Glycoproteins
  • NK Cell Lectin-Like Receptor Subfamily A
  • RNA, Messenger
  • Receptors, Immunologic
  • Receptors, NK Cell Lectin-Like
  • Concanavalin A