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J Rheumatol. 1997 Sep;24(9):1774-9.

Suppression of matrix metalloproteinase-3 synthesis by interleukin-4 in human articular chondrocytes.

Author information

1
Department of Orthopaedic Surgery, National Defense Medical College, Saitama, Japan.

Erratum in

  • J Rheumatol 1998 Jan;25(1):193. Shimmei M [corrected to Shinmei M].

Abstract

OBJECTIVE:

To evaluate the effect of interleukin-4 (IL-4) on IL-1 induced matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinase-1 (TIMP-1) production by human articular chondrocytes.

METHODS:

Monolayer cell culture of chondrocytes was obtained from human articular cartilage from patella within 24 h after death. MMP-3 and TIMP-1 protein levels were determined by ELISA. MMP-3 activity was assayed as caseinase activity. Amounts of MMP-3 and TIMP-1 mRNA were measured by Northern blot analysis.

RESULTS:

IL-4 suppressed IL-1 stimulated MMP-3 protein and enzyme activity. Moreover, IL-4 suppressed IL-1 induced MMP-3 mRNA. In contrast, IL-4 did not alter the level of TIMP-1 protein and mRNA.

CONCLUSION:

IL-4 may be implicated as a protective mediator of joint destruction seen in inflammatory arthritis.

PMID:
9292803
[Indexed for MEDLINE]

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