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Fungal Genet Biol. 1997 Jun;21(3):315-22.

Mapping chromosome landmarks in the centromere I region of Neurospora crassa.

Author information

1
Departamento de Químice Biológica (CIQUIBIC-CONICET), Faculted de Ciencias Químicas, Universidad Nacional de Córdoba, Argentina. alberto@dqbfeq.uncoredu

Abstract

Chromosome translocation breakpoints, RFLP heterozygosity in partial chromosome duplications, and RFLP-marked crossover events have been used as chromosomal landmarks to find the position and orientation of cloned regions flanking centromere I of Neurospora crassa. Determination of physical:genetic ratios in genomic regions flanking the loci mei-3, un-2, and his-2 supports previous evidence indicating that recombinational activity is lower in regions flanking centromere I than in the general N. crassa genome. The homogeneous distribution of crossover events found in these regions suggests that there is not a gradient of crossover inhibition in the vicinity of centromere I. Thus, a largely extended centromeric effect and/or a general crossover inhibitory effect operating on linkage group I (LGI) could constitute the basis of these abnormal physical:genetic ratios. A DNA element containing about 76% A+T was isolated from the centromeric end of a cloned region on LGIR. The fragment includes a previously undescribed DNA sequence, highly repeated in the Neurospora genome, which may correspond to centromeric DNA.

PMID:
9290244
DOI:
10.1006/fgbi.1997.0987
[Indexed for MEDLINE]

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