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FEBS Lett. 1997 Aug 18;413(2):194-6.

Unexpected influence of a C-terminal-fused His-tag on the processing of an enzyme and on the kinetic and folding parameters.

Author information

1
Centre for Protein Engineering, Université de Liège, Institut de Chimie, Belgium.

Abstract

The addition of a poly-His C-terminal extension, designed to facilitate the purification of the protein, to the beta-lactamase of a thermophilic Bacillus licheniformis strain modified the site of action of the signal peptidase. This resulted in the secretion of a protein with a different N-terminus, showing that this type of protein engineering might not always be as 'neutral' as generally assumed.

PMID:
9280280
DOI:
10.1016/s0014-5793(97)00908-3
[Indexed for MEDLINE]
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