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Nucleic Acids Res. 1997 Sep 15;25(18):3749-50.

Multiplex genotyping of PCR products with MassTag-labeled primers.

Author information

1
PerSeptive Biosystems, Inc., 500 Old Connecticut Path, Framingham, MA 01701, USA. lhaff@pbio.com

Abstract

A simple mass spectrometric based assay, the PinPoint assay, has previously been described for typing single nucleotide polymorphisms. The identity of a polymorphism is determined by mass differences of single base extended genotyping primers as determined by MALDI-TOF mass spectrometry. A simple method for multiplexing the assay is described, employing multiple primers with 5'oligo(dT) sequences (MassTags) which serve to mass discriminate the peaks of multiple extended and non-extended primers. The assay is extremely rapid and requires no labeling reagents.

PMID:
9278502
PMCID:
PMC146949
[Indexed for MEDLINE]
Free PMC Article

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