Na+/Ca2+ exchange current and contractions measured under Cl(-)-free conditions in developing rabbit hearts

Am J Physiol. 1997 Aug;273(2 Pt 2):H837-46. doi: 10.1152/ajpheart.1997.273.2.H837.

Abstract

Previous studies suggesting a greater functional role of cardiac Na+/Ca2+ exchange at birth were performed using tightly buffered free cytosolic Ca2+ concentration ([Ca2+]i). Because Na+/Ca2+ exchange current (INaCa) is influenced by physiological fluctuations in [Ca2+]i, we used conditions of minimally buffered [Ca2+]i to simultaneously record INaCa and cell contractions in single ventricular myocytes isolated from 1 to 27-day-old and adult rabbits. With conventional Cl(-)-containing solutions. Ni(2+)-sensitive outward and inward charge movements were unbalanced, suggesting the presence of a contaminating current (presumably the Ca(2+)-activated Cl- current). Removing Cl- abolished this discrepancy in all age groups and allowed for the accurate quantitation of INaCa. Under Cl(-)-free conditions, outward and inward charge movements were high at birth (4 days: 0.42 +/- 0.03 and -0.38 +/- 0.04 pC/pF, respectively) and decreased postnatally (adult: 0.08 +/- 0.01 and -0.07 +/- 0.01 pC/pF, respectively). Newborn but not adult myocytes contracted during depolarizations in the presence of nifedipine, ryanodine, and thapsigargin. The magnitudes of outward charge movement (Ca2+ influx) and cell shortening exhibited similar voltage dependence, consistent with INaCa-mediated contractions. These results indicate that INaCa can directly support contraction in newborn rabbit ventricular myocytes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aging / metabolism
  • Animals
  • Carrier Proteins / drug effects
  • Carrier Proteins / physiology*
  • Cell Separation
  • Chlorides / pharmacology*
  • Chlorides / physiology
  • Electric Conductivity
  • Electrophysiology
  • Myocardial Contraction* / drug effects
  • Myocardium / cytology
  • Myocardium / metabolism*
  • Nickel / pharmacology
  • Rabbits
  • Reaction Time
  • Sodium-Calcium Exchanger
  • Solutions

Substances

  • Carrier Proteins
  • Chlorides
  • Sodium-Calcium Exchanger
  • Solutions
  • Nickel