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FEBS Lett. 1997 Aug 4;412(3):633-6.

K+ is an indispensable cofactor for GrpE stimulation of ATPase activity of DnaK x DnaJ complex from Thermus thermophilus.

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1
Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Yokohama, Japan.

Abstract

K+ is an indispensable cofactor for ATPase activity of eukaryotic cytosolic Hsp70 chaperone systems which lack a GrpE homolog. In the case of the bacterial Hsp70 (DnaK) system, GrpE, a nucleotide exchange factor, stimulates ATPase activity but little is known about the effect of K+. Here, we have cloned a grpE gene from a thermophile, Thermus thermophilus, and purified a homodimeric GrpE protein. Using proteins of this bacterium, we found that the GrpE stimulation of ATPase activity of DnaK x DnaJ complex was absolutely dependent on the presence of K+.

PMID:
9276481
DOI:
10.1016/s0014-5793(97)00847-8
[Indexed for MEDLINE]
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