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Parasitol Res. 1997;83(7):719-21.

A new method for isolation and differentiation of native Entamoeba histolytica and E. dispar cysts from fecal samples.

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Institut für Tropenmedizin, Forschungslaboratorien, Tübingen, Germany.


A new method for the purification of protozoan cysts from feces was established, allowing to isolation of native cysts. The procedure consists of two sucrose-density gradients and enzymatic digestion of cellulose particles by cellulase and can be accomplished in a few hours. The cyst fractions were differentiated into Entamoeba histolytica and E. dispar using the DNA probes P145 and B133 and a dot-blot test.

[Indexed for MEDLINE]

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