Send to

Choose Destination
See comment in PubMed Commons below
Curr Opin Biotechnol. 1997 Aug;8(4):488-97.

Rapid, sensitive sequencing of oligosaccharides from glycoproteins.

Author information

Department of Biochemistry, University of Oxford, UK.


The release of sub-picomole levels of N-linked oligosaccharides directly from 1-5 micrograms of protein in a band on an SDS PAGE gel, coupled with recent advances in mass spectrometry and HPLC, opens the way for the analysis of biologically important glycoproteins that are difficult to purify or are available only in limited amounts. A straightforward HPLC strategy enables the structures of both neutral and sialylated components of the N-glycan pool to be predicted from a single run. The entire pool of sugars may then be sequenced simultaneously, using exoglycosidase arrays.

[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center