Cell cycle specificity of Fas-mediated apoptosis in WIL-2 cells

FEBS Lett. 1997 Jul 21;412(1):91-3. doi: 10.1016/s0014-5793(97)00740-0.

Abstract

Antibodies to Fas/APO1 receptor induce effective apoptosis in WIL-2 cells of the human B-lymphoid line. Quantitative assessment of the extent of the death in cells synchronized by thymidine block revealed a significant increase in their sensitivity to the cytocidal effect mediated by Fas/APO1 during the G1 phase of the cell cycle. Western analysis of the content of the p53 antigen in the cytoplasm and nuclei of the cells showed that the Fas/APO1-induced death is accompanied by massive translocation of the p53 from the cytoplasm to the nucleus. These findings suggest that cell vulnerability to the Fas/APO1-mediated apoptosis is subjected to regulation by cell cycle-dependent mechanisms, one of which is probably the function of the p53 antigen.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies / pharmacology
  • Apoptosis*
  • B-Lymphocytes / cytology*
  • Blotting, Western
  • Cell Cycle*
  • Cell Line
  • Cell Nucleus / metabolism
  • Cytoplasm / metabolism
  • G1 Phase
  • Humans
  • Kinetics
  • Mimosine / pharmacology
  • Tumor Suppressor Protein p53 / metabolism
  • fas Receptor / immunology
  • fas Receptor / physiology*

Substances

  • Antibodies
  • Tumor Suppressor Protein p53
  • fas Receptor
  • Mimosine