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FEBS Lett. 1997 Jul 21;412(1):39-42.

ATR-FTIR spectroscopic investigation of E. coli transconjugants beta-lactams-resistance phenotype.

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Laboratoire de Spectroscopie Biomoléculaire, IFR 53, UFR de Pharmacie,Université de Reims Champagne-Ardenne, France.


Hyphenation of attenuated total reflection Fourier transform infrared spectroscopy and cluster analysis has been used to characterise a susceptible Escherichia coli K12 strain and the transconjugants TEM-1, TEM-2, TEM-3, SHV-2, SHV-3, SHV-4. A good discrimination of the susceptible strain from the transconjugants was obtained. Although a limited success was achieved in the differentiation of SHV and TEM phenotypes in general, results obtained with TEM-2 and SHV-3 were convincing. Spectral differences observed are ascribed to the global effects of the conjugation process, particularly their repercussions in the nucleic acids and carbohydrate absorbing regions, rather than to beta-lactamase point-mutations.

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