Effects of transforming growth factor-beta on the production of parathyroid hormone-related peptide in a human ovarian cancer cell line in vitro

J Obstet Gynaecol Res. 1997 Jun;23(3):231-8. doi: 10.1111/j.1447-0756.1997.tb00837.x.

Abstract

Objective: We examined the effects of transforming growth factor-beta 1 (TGF-beta 1) on intracellular parathyroid hormone-related peptide (PTHrP) production in a human ovarian cancer cell line (HOC-21).

Methods: Various concentrations of TGF-beta 1 were added to a culture medium, and the numbers of cells were counted. HOC-21 cells were cultured with or without TGF-beta 1, and intracellular PTHrP was measured by a radioimmunoassay kit that recognized the carboxy-terminal protein while counting the number of cells. Furthermore, a 125I-TGF-beta 1 binding assay was carried out. Values were analyzed statistically using an analysis of variance followed by an unpaired t-test.

Results: Five ng/ml of TGF-beta 1 inhibited the cell growth on Day 1 after plating. The TGF-beta 1 significantly (p < 0.05) stimulated intracellular PTHrP production in a time-dependent manner, also by Day 1. A 125I-TGF-beta 1 binding study revealed that HOC-21 cells expressed a high affinity for the TGF-beta 1 receptor.

Conclusion: The suppression of human ovarian cancer cell proliferation by TGF-beta 1 might be involved in the production of PTHrP.

MeSH terms

  • Cell Division
  • Culture Media
  • Cystadenocarcinoma, Papillary / metabolism
  • Cystadenocarcinoma, Serous / metabolism
  • Female
  • Humans
  • Middle Aged
  • Ovarian Neoplasms / metabolism*
  • Parathyroid Hormone-Related Protein
  • Protein Biosynthesis*
  • Transforming Growth Factor beta / pharmacology*
  • Tumor Cells, Cultured

Substances

  • Culture Media
  • PTHLH protein, human
  • Parathyroid Hormone-Related Protein
  • Transforming Growth Factor beta