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Anticancer Res. 1997 Jul-Aug;17(4A):2827-30.

Immunohistochemical analysis of 4-aminobiphenyl-DNA adducts in oral mucosal cells of smokers and nonsmokers.

Author information

1
Institute of General Pathology, Centro di Ricerche Oncologiche Giovanni XXIII, Catholic University School of Medicine, Rome, Italy.

Abstract

BACKGROUND:

The "biologically effective dose markers", DNA and protein adducts, are a direct index of carcinogen induced cell damage and an indirect one of genetic susceptibility. This study aimed to examine the dose-response relationship for 4-Aminobiphenyl-DNA adducts in oral cells of smokers and non smokers.

MATERIALS AND METHODS:

An immunoperoxidase method with the monoclonal 3C8 antibody, which recognizes 4-Aminobiphenyl-DNA, has been used for detecting DNA damage in oral cells of 12 smokers and 12 non smokers.

RESULTS:

Higher staining for 4-Aminobiphenyl-DNA was detected in the cells of smokers (187 +/- 42) vs. non smokers (135 +/- 35) (p = 0.004), with a twofold range in relative staining for both groups, suggesting individual differences relevance in metabolizing carcinogens and/or repairing DNA damage.

CONCLUSIONS:

This non invasive method requiring small cell amounts is a tool for monitoring large groups of subjects at risk in primary prevention programs.

PMID:
9252724
[Indexed for MEDLINE]

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