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Anticancer Res. 1997 Jul-Aug;17(4A):2471-4.

A model for osseous metastasis of human breast cancer established by intrafemur injection of the MDA-MB-435 cells in nude mice.

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1
Department of Urology, State University of New York Health Science Center, Syracuse 13210, USA.

Abstract

BACKGROUND:

A nude mouse model was developed and studied for temporal pathogenesis of bony metastasis of human breast cancer.

MATERIALS AND METHODS:

Human breast cancer cells, MDA-MB-435, were injected into the medulla of left femurs of 10 Beige nude mice, whereas their right femurs were similarly injected with phosphate buffered saline as a control. The mice were killed at 1 week-intervals for up to 5 weeks.

RESULTS:

Tumors appeared in all the animals 3 weeks after the injection: they began to appear in the medulla at 2 weeks, and invasion of the tumor into the cortex and surrounding tissues of the bone appeared at 4 weeks. Both osteolytic and osteoblastic activation frequently observed in the affected bone of breast cancer patients were present.

CONCLUSION:

This model produces a high incidence of bony metastasis and may be appropriate for the study of osseous metastasis of this human disease.

PMID:
9252665
[Indexed for MEDLINE]
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