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J Antimicrob Chemother. 1997 Jul;40(1):109-12.

Amphotericin B resistance testing of Candida spp.: a comparison of methods.

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Department of Microbiology, University of Manchester, Hope Hospital, Salford, UK.


Several methods of susceptibility testing were compared for their ability to detect amphotericin B resistance among yeast isolates, including isolates known to be amphotericin-B-resistant in an animal model. A microtitre MIC method using antibiotic medium no. 3 and Etest strips were able to discriminate susceptible and resistant isolates. A disc susceptibility test was also able to identify resistant isolates and this method may be suitable for routine use as a screening test. A survey of resistance in 644 clinical isolates showed that the incidence of elevated MICs (> or = 0.25 mg/L) was only 1.2%.

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