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Gene. 1997 Jul 1;193(1):119-25.

Cloning and expression of human NF-YC.

Author information

1
Dipartimento di Genetica e Biologia dei Microrganismi, Università di Milano, Milan, Italy.

Abstract

The CCAAT box is an important element in eukaryotic promoters and NF-Y (CBF) is a conserved heterotrimeric protein binding to it. Two subunits, NF-YB and NF-YC, contain a histone-like motif. We cloned the complete cDNA coding for the human NF-YC gene. The ORF codes for a 335 aa protein that shows virtual identity to the rat sequence, confirming the stunning invariance of NF-Y genes across species. We expressed and purified the yeast homology domain of NF-YC in bacteria and performed EMSA together with the corresponding conserved domains of NF-YA and NF-YB, obtaining a CCAAT-binding mini-NF-Y. We evaluated the expression of NF-YC and found that mRNA levels are similar in different human tissues except in testis.

PMID:
9249075
DOI:
10.1016/s0378-1119(97)00109-1
[Indexed for MEDLINE]

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