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Anal Biochem. 1997 Aug 1;250(2):203-11.

Manual manufacturing of oligonucleotide, DNA, and protein microchips.

Author information

1
Joint Human Genome Program, Argonne National Laboratory, Illinios 60439, USA.

Abstract

A simple procedure for manufacturing microchips containing various gel-immobilized compounds is described. A gel photopolymerization technique is introduced to produce micromatrices of polyacrylamide gel pads (25 x 25 x 20 microm and larger) separated by a hydrophobic glass surface. A pin device for the manual application of a compound in solution onto the activated polyacrylamide gel pad for immobilization is described. Oligonucleotide, DNA, and protein microchips have been produced by this method and tested by hybridization and immunoanalysis monitored with a fluorescence microscope. The effect of the lengths of the immobilized oligonucleotides and the hybridized RNA and DNA on hybridization of the oligonucleotide microchips was evaluated. This method can also be used for manufacturing microchips containing a variety of other compounds.

PMID:
9245440
DOI:
10.1006/abio.1997.2209
[Indexed for MEDLINE]

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