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Cell. 1997 Jul 25;90(2):335-49.

Bidirectional transport by distinct populations of COPI-coated vesicles.

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1
Department of Morphology, Faculty of Medicine, University of Geneva Medical Center, Switzerland.

Abstract

Electron microscope immunocytochemistry reveals that both anterograde-directed (proinsulin and VSV G protein) and retrograde-directed (the KDEL receptor) cargo are present in COPI-coated vesicles budding from every level of the Golgi stack in whole cells; however, they comprise two distinct populations that together can account for at least 80% of the vesicles budding from Golgi cisternae. Segregation of anterograde- from retrograde-directed cargo into distinct sets of COPI-coated vesicles is faithfully reproduced in the cell-free Golgi transport system, in which VSV G protein and KDEL receptor are packaged into separable vesicles, even when budding is driven by highly purified coatomer and a recombinant ARF protein.

PMID:
9244307
DOI:
10.1016/s0092-8674(00)80341-4
[Indexed for MEDLINE]
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