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FEBS Lett. 1997 Jun 30;410(2-3):275-9.

Purification and structure of mutacin B-Ny266: a new lantibiotic produced by Streptococcus mutans.

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1
Centre de Recherche en Sciences et Technologie du Lait (STELA), Faculté des Sciences de l'Agriculture et de l'Alimentation, Université Laval, Québec, Canada.

Abstract

Mutacins are bactericidal substances of proteinaceous nature produced by Streptococcus mutans. Lantibiotics are antibacterial substances containing post-translationally modified amino acids such as lanthionine. Mutacin B-Ny266 was purified from the cell pellet of S. mutans strain Ny266 by ethanol extraction at pH 2.0 followed by reversed-phase chromatography (Sep-Pak cartridge) and by HPLC on a C18 column. The mean purification factor was 3240 +/- 81 and the mean yield was 1.0 +/- 0.1%. Molecular mass of mutacin B-Ny266 as determined by mass spectroscopy is 2270.29 +/- 0.21 Da. The amino acid sequence of the purified active fraction was obtained by Edman degradation after treatment with alkaline ethanethiol. Twenty-one amino acids were detected in this analysis. Mutacin B-Ny266 belongs to the type A lantibiotics. The proposed sequence is: F-K-A-W-U-F-A-Abu-P-G-A-A-K-O-G-A-F-N-U-Y-A. The molecule differs from that of epidermin/staphylococcin 1580 and gallidermin at positions 1, 2, 4, 5 and 6.

PMID:
9237644
DOI:
10.1016/s0014-5793(97)00425-0
[Indexed for MEDLINE]
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