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FEMS Microbiol Lett. 1997 Jul 15;152(2):243-7.

Localization of germination-specific spore-lytic enzymes in Clostridium perfringens S40 spores detected by immunoelectron microscopy.

Author information

1
Department of Applied Biological Sciences, School of Agricultural Sciences, Nagoya University, Aichi, Japan.

Abstract

The localization of germination-specific spore-lytic enzymes, an amidase and a muramidase, in Clostridium perfringens S40 spores was examined by immunoelectron microscopy with respective antisera raised against the enzymes and a colloidal gold-immunoglobulin G complex. For both antisera, immunogold particles were visualized on the outside of the cortex of dormant spores, and they were not detected in germinated spores and decoated spores.

PMID:
9231416
DOI:
10.1016/s0378-1097(97)00204-8
[Indexed for MEDLINE]
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