Format

Send to

Choose Destination
Can J Microbiol. 1997 Jun;43(6):534-40.

Recombinant plasmid mobilization between E. coli strains in seven sterile microcosms.

Author information

1
Observatoire océanologique, Unité de recherche associée, Centre national de la recherche scientifique 2071, Banyuls-sur-mer, France. lebaron@arago.univ-perp.fr

Abstract

Transfer by mobilization of a pBR derivative recombinant plasmid lacking transfer functions (oriT+, tra-, mob-) from one E. coli K12 strain to another was investigated in seven sterile microcosms corresponding to different environments. These microcosms were chosen as representative of environments that genetically engineered microorganisms (GEMOs) encounter after accidental release, namely attached biomass in aquatic environments (biofilm), soil, seawater, freshwater, wastewater, mouse gut, and mussel gut, GEMOs survived in the same way as the host strains in all microcosms. Recombinant DNA mobilization occurred in the mouse gut, in sterile soil, and in biofilm. The plasmid transfer rates principally reflected the environmental conditions encountered in each microcosm.

PMID:
9226873
DOI:
10.1139/m97-076
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Atypon
Loading ...
Support Center