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J Pept Sci. 1996 Mar-Apr;2(2):125-33.

Solid-phase synthesis and cellular localization of a C- and/or N-terminal labelled peptide.

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1
Centre de Recherche de Biochimie Macromoléculaire, Montpellier, France.

Abstract

We report the solid-phase synthesis by the Fmoc strategy of a peptide containing a cysteamide group at its C-terminus. This peptide was subjected to further modifications including the linkage of fluorophores, namely lucifer yellow and coumarin respectively, at the C- and/or N-terminals. After incubation with living cultured cells these two probes were localized and it is concluded that the post-synthesis modifications can strongly modify the localization of the peptide.

PMID:
9225252
DOI:
10.1002/psc.58
[Indexed for MEDLINE]
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