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Nat Biotechnol. 1997 Jul;15(7):668-72.

Immunoglobulin-like domains define the nerve growth factor binding site of the TrkA receptor.

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Department of Medicine (Care of the Elderly), Bristol Royal Infirmary, UK.


Nerve growth factor (NGF) is involved in the development and maintenance of the nervous system. NGF binds with high affinity to the extracellular region of the tyrosine kinase receptor TrkA. This domain comprises leucine and cysteine rich motifs, followed by two immunoglobulin like (Ig-like) domains. We describe the expression and purification of recombinant Ig-like domains. Fluorescence and circular dichroism spectroscopy show that the protein is folded into a compact globular structure and contains mainly beta-sheet secondary structure. Recombinant protein binds to NGF and can inhibit NGF bioactivity both in vitro and in vivo.

[Indexed for MEDLINE]

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