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Cell. 1997 Jun 27;89(7):1067-76.

Daxx, a novel Fas-binding protein that activates JNK and apoptosis.

Author information

1
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.

Abstract

The Fas cell surface receptor induces apoptosis upon receptor oligomerization. We have identified a novel signaling protein, termed Daxx, that binds specifically to the Fas death domain. Overexpression of Daxx enhances Fas-mediated apoptosis and activates the Jun N-terminal kinase (JNK) pathway. A C-terminal portion of Daxx interacts with the Fas death domain, while a different region activates both JNK and apoptosis. The Fas-binding domain of Daxx is a dominant-negative inhibitor of both Fas-induced apoptosis and JNK activation, while the FADD death domain partially inhibits death but not JNK activation. The Daxx apoptotic pathway is sensitive to both Bcl-2 and dominant-negative JNK pathway components and acts cooperatively with the FADD pathway. Thus, Daxx and FADD define two distinct apoptotic pathways downstream of Fas.

PMID:
9215629
PMCID:
PMC2989411
DOI:
10.1016/s0092-8674(00)80294-9
[Indexed for MEDLINE]
Free PMC Article

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