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Eur J Biochem. 1997 Jun 1;246(2):388-93.

An ADP-ribosylation-factor(ARF)-like protein involved in regulated secretion.

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Laboratoire de Génétique Moléculaire de la Neurotransmission, C.N.R.S., Hôpital de la Pitié-Salpêtrière, Paris, France.


A rat ADP-ribosylation factor(ARF)-like protein named ARL184 was identified by cDNA cloning. The corresponding recombinant protein had an apparent molecular mass of 22,000. The deduced amino acid sequence had 55% identity with the human ARL1 and four functional GTP-binding sites. Immunofluorescent confocal microscopy studies showed that ARL184 was present in the cytosol as well as in the Golgi apparatus, raising the possibility that it has a role in a secretory pathway. The involvement of this ARF-like protein in secretion was confirmed by demonstrating that ARL184 potentiated acetylcholine release in stably transfected PC12 cells. Collectively these results suggest that this ARL protein is a component of a regulated secretory pathway involved in Ca2(+)-dependent release of acetylcholine.

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