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Nucleic Acids Res. 1997 Jul 15;25(14):2938-9.

A universal procedure for primer labelling of amplicons.

Author information

1
School of Microbiology and Immunology, The University of New South Wales, Sydney, NSW 2052, Australia. b.neilan@unsw.edu.au

Abstract

Detection and visualisation of nucleic acids is integral to genome analyses. Exponential amplification procedures have provided the means for the manipulation of nucleic acid sequences, which were otherwise inaccessible. We describe the development and application of a universal method for the labelling of any PCR product using a single end-labelled primer. Amplification was performed in a single reaction with the resulting amplicon labelled to a high specific activity. The method was adapted to a wide range of PCRs and significantly reduced the expense of such analyses.

PMID:
9207046
PMCID:
PMC146818
[Indexed for MEDLINE]
Free PMC Article
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